MEET THE OT-2
Lab automation has never been easier
The high-precision OT-2 lab robot is flexible, open-source, and user-friendly.
Ideal for:
- Genomics
- Proteomics
- Cell-based assays
- Biochemical assays
- Drug discovery
flexible hardware
One robot, countless workflows
Configurable 11-deck platform with options for Magnetic, Temperature and Thermocycler Modules
Swappable single & 8-channel pipettes for multiple volume configurations
Standardized (SBS) deck slots allow you to use labware you already own
Performance
Accuracy: 15% at 1uL
Precision: 5% at 1uL
Throughput: Fast fill a 96-well plate in 22 seconds
flexible software
Powerful software at your fingertips
Choose from free, beginner-friendly & advanced protocol controls
Powerful cloud-based tools
Unmatched control of nearly every aspect of your workflow
User Friendly
Self-serve unboxing and installation
Reagent and bead system agnostic
Plug-and-play setup with access to protocol libraries to enable immediate assay implementation
Easily integrates to your existing instruments
Compatible with ANSI/SBS compliant or automation friendly tubes, microplates, deep-well plates, reservoirs and adapters
open source
Near limitless control
100% customizable and open to modifications adjustable to your assay
Modify or customize protocols with our simple yet powerful Python API
Digital protocol commands surpass manual pipetting capabilities
Updates
Supports Ethernet or USB connection (external dongle provided)
4 USB ports available to support connection to multiple modules
Easy access to electronics via external PCBA enclosure
LED indicator provide users feedback, diagnosis & troubleshooting aid on instrument power state, system processing, ethernet, and network connectivity
Beginner-Friendly
So easy, you’ll feel like an automation expert
Real-time chat support on your computer or mobile phone.
Plug-and-play. Unbox, set-up, calibrate. No technician needed.
Dedicated Account Managers available to you at every step.
Start with confidence. Demo the OT-2 for 60 days.
Specs
Lab automation rethought from the ground up
- Compact footprint
- 63cm x 57cm x 66cm / 25in x 22.5in x 26in (W,D,H)
- Takes up half of your standard lab bench
- Pipette configurations
- Single and 8-channel pipetting
- 2-pipette mounts, for a configuration of 1 or 2 single or 8-channel pipettes. Pipettes are easily interchangeable.
- Pipette volumes and specs
- Single channel: 1-1000µl
- 8-channel: 1-300µl
- Opentrons Pipette Whitepaper (GEN1 & GEN2) ›
- Single channel manual ›
- 8-channel manual ›
- Deck
- 11 deck slots enable countless configurations
- Deck slots are compatible with standard SBS dimensions. Deck also includes a removable trash bin.
- Connectivity
- WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0
- Learn more ›
- Weight
- 48kg or 105.8 lb
- Video
- Integrated video camera (Included for future or custom functionality)
- Audio
- Integrated audio system (Included for future or custom functionality)
- Power Requirements
- Robot Power Input: 36VDC, 6.1A
- Power Adapter Input: 100-240VAC, 50/60Hz, 4.0A/115VAC, 2.0A/230VAC
- For additional power requirement information, please review this support article
- Frame composition
- Rigid steel and CNC aluminum design
- Operating Temperature & Relative Humidity
- Recommended 20-24C Recommended 40-60% RH
- Learn more ›
Speed
Fills a 96-well plate in 22 seconds
| Pipette | Distribute, One Tip | Transfer, Same Tip | Transfer, New Tip |
|---|---|---|---|
P20 8-channel | 0:22 | 1:35 | 4:19 |
P300 8-channel | 0:26 | 1:38 | 4:24 |
P20 Single | 3:12 | 13:10 | 36:01 |
P300 Single | 4:01 | 13:39 | 41:31 |
P1000 Single | 5:54 | 15:21 | 44:50 |
Accuracy and precision
Single and 8-channel pipetting
| Pipette | Volume | Random Error | Systematic Error | ||
|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||
| p20 | 1 | ±15% | 0.15µL | ±5% | 0.05µL |
| 10 | ±2% | 0.2µL | ±1% | 0.1µL | |
| 20 | ±1.5% | 0.3µL | ±0.8% | 0.16µL | |
| p300 | 20 | ±4% | 0.8µL | ±2.5% | 0.5µL |
| 150 | ±1% | 1.5µL | ±0.4% | 0.6µL | |
| 300 | ±0.6% | 1.8µL | ±0.3% | 0.9µL | |
| p1000 | 100 | ±2% | 2.0µL | ±1% | 1µL |
| 500 | ±1% | 5.0µL | ±0.2% | 1µL | |
| 1000 | ±0.7% | 7.0µL | ±0.15% | 1.5µL | |
| Pipette | Volume | Random Error | Systematic Error | ||
|---|---|---|---|---|---|
| %D | µl | %CV | µl | ||
| P20 | 1 | ±20% | 0.2µL | ±10% | 0.1µL |
| 10 | ±3% | 0.3µL | ±2% | 0.2µL | |
| 20 | ± 2.2% | 0.44µL | ±1.5% | 0.3µL | |
| P300 | 20 | ±10% | 2.0µL | ±4% | 0.8µL |
| 150 | ±2.5% | 3.75µL | ±0.8% | 1.2µL | |
| 300 | ±1.5% | 4.5µL | ±0.5% | 1.5µL | |
Sterility
Full polycarbonate enclosure designed to limit exposure. The OT-2 is not a sterile environment
Add the Opentrons HEPA Module, or add your own UV lights
Viscosity
For best practices on automating viscous liquid handling, review our application note
- API compatibility
- Interfaces with any product that has an API
- Opentrons software is open-source and users can make adjustments to the software as needed to interface with other products. While all integrations are possible, they all require custom code work i.e. most customers will need to write custom libraries and install software packages that are not shipped with the robot.
- Learn more ›
- Interface
- Windows 10, macOS 10.10 or later, Ubuntu 12.04 or later.
- Opentrons App ›
- Tip compatibility
- Use Opentrons Tips and Opentrons Filter Tips
- Opentrons guarantees the performance of Opentrons Tips which were specifically designed for the OT-2. The OT-2 was designed to accommodate all universal bore tip types, but tip quality and dimensions can vary significantly so third party tip performance cannot be guaranteed by Opentrons.
- Labware compatibility
- Use common labware already in our Labware Library, or add your own
- If your labware fits on the OT-2 but is not in the Labware Library, it can be added by you using our Labware Creator, or by our team.
- Learn more ›
- OT-2 Manual ›
- Warranty
- One year warranty against manufacturer defects
- Stress tested to last a minimum of 3 years of full-time use
- Learn more ›
- Return Policy
- 60 day, 100% risk free
- Learn more ›
- Certifications
- Certifications complete (CE, FCC)
- Certifications pending (NRTL, CB)
- Not certified/validated (IVD, GMP, ISO)
- Pick up tip
- Drop tip
- Return tip
- New tip (always, never, once)
- More...
- Aspirate
- Dispense
- Aspirate speed
- Dispense speed
- Blowout
- Touch tip
- Mix
- Air gap
- Move to
- Delay
- User-specified pause
- Homing
- Comment
- Basic transfer
- Volumes > Pipette Volume
- Distribute
- Consolidate
- Speed
- Volume
- Count
- Dispose
Welcome To Opentrons!
How The Opentrons Platform Works
Custom Modular Hardware
Setting Up Your OT-2
OT-2 Hardware Video
Risk free 60-day demo
Try the OT-2 in your lab today and take advantage of protocols designed for sharing and editing.
